Women are 4 to 6 times more likely to sustain an anterior cruciate ligament (ACL) injury than men. Women with ACL reconstruction (ACLR) are also 2.36 times less likely to be active than healthy individuals. In addition to reductions in physical activity (PA), it has been reported that BMI remains elevated up to 2 years post-ACLR, but the effects of ACLR on body composition profiles following surgery is unclear. PURPOSE: To compare PA engagement and body composition between women with ACLR and healthy controls. METHODS: Ten women with ACLR (less than 5yrs post-ACLR, age=21.4±3.8yrs, BMI=26.1±3.8kg/m²) and 10 healthy women (age=21.9±3.1yrs, BMI=21.8±2.5kg/m²) completed the Tegner Activity Scale to assess current PA level. Body fat percentage (%BF) was estimated using air displacement plethysmography. Objective PA was assessed using hip-worn accelerometers for 10 hours/day for 7 days and Freedson Adult VM3 cut points were used for PA data processing. Groups were compared using Mann-Whitney U tests due to limited sample size. Time (minutes/week) spent in moderate-to-vigorous PA (MVPA) and step count (steps/day) were compared between groups using a one-way ANCOVA with total wear time as a covariate. RESULTS: Women with ACLR had significantly higher %BF than controls (ACLR=32.7±6.7%, healthy=22.6±4.9%; p<0.01) and higher fat mass (ACLR=25.4±9.0kg, healthy=13.7±4.1kg; p<0.01). Healthy women participated in significantly more steps per day (ACLR=6650±3227 steps/day, healthy=9361±2626 steps/day; p=0.02) than women with ACLR, but no differences in MVPA time (ACLR=367.2±226.2 mins/week, healthy=448.9±164.0 mins/week; p=0.34) were observed. CONCLUSIONS: Women with ACLR engage in similar levels of MVPA compared to controls, but they may exhibit greater %BF and fat mass than women who have not experienced ACLR. Compared to controls, women with ACLR also have lower step counts, which is consistent with previous studies. Lack of PA, high %BF, and overweight status increase the risk of premature mortality and morbidity. Therefore, it is crucial to further assess these characteristics and determine potential interventions that would promote PA and maintenance of healthy body composition in the months following ACLR.