<PURPOSE: evaluating the effects of Liraglutide over the adipose tissue of Swiss mice subjugated to a cafeteria diet and physical activity, through swimming. METHODS: approved by the Ethics in Animal Use of UFRN (n. 003/2014). We have divided 74 animals (Swiss mice) into two phases: the initial phase had a pilot study (n=10) subdivided into the control group (PCON) (n=5) and the cafeteria group (PCAF) (n=5), to evaluate an attractive cafeteria diet to the animals taste, that would result in an increase of body weight. After the diet's introduction, the animals were weighed, euthanized, and had their intra-abdominal adiposity measured. The menu offered to the group PCAF was deemed attractive to taste and chosen for the development of the research. After, 64 animals were subdivided into two groups: the Cafeteria Base Study Group (EBCAF), further subdivided: cafeteria+exercise+liraglutide (CEL) (n=8), cafeteria+exercise+saline (CES) (N=8), cafeteria+liraglutide (CL) (n=8), and cafeteria+saline (CS) (n = 8). And the Chow Base Study Group (EBR), that was further subdivided: exercise+liraglutide (EL) (n=8), exercise+saline (ES) (n=8), liraglutide (L) (n=8), and saline solution (SS) (n = 8). All the animals were put through a cafeteria diet, following the physical activity with swimming and treating them with testing substances intraperitoneally (200μg/mL/kg). RESULTS: In the pilot study, the type of food did not significantly affect the increase in weight gain (PCAF = 47.38±0.70 vs. PCON = 46.48±1.08). However, it changed considerably the increase of intraabdominal adipose tissue: CAF = 0.74±0.05 vs. CON = 0.44 ± 0.08 g (p < 0.05). The base study of the research showed that the treatment with liraglutide associated with physical activity when compared to saline had a significant reduction in the mass of adipose tissue: CEL-0.32±0.03 vs. CES-0.48±0.05 g; CL-0.41±0.09 vs. CS-0.76±0.09 g; L-0.24±0.04 vs. SS-0.52±0.08 g e CEL-0.32±0.03 vs. CS-0.76±0.09 g (p < 0.05 CEL vs. CES; p < 0.05 CL vs. CS; p < 0.01 L vs. SS; p < 0.001 CEL vs. CS.) (0.32 ± 0,05 g), compared to the saline group (0.53±0.07 g).
CONCLUSIONS: the liraglutide supplied a weight loss in animals, especially in the intraabdominal adipose tissue and when associated with Physical activity has allowed greater efficiency in terms of adipose tissue decrease.>