Whole-body vibration (WBV) exposes the entire body to mechanical oscillations when one is standing on a vibrating platform. In recent years, researchers have studied the extent to which these oscillations effect the body. Previous studies have investigated muscle activity in the general population during static exercises with WBV, but there has been little research that has focused on the effects of WBV during dynamic movements in athletes. PURPOSE: The purpose of the study was to investigate the effects of WBV on rectus femoris muscle activity during a whole-body squat (WBS) exercise in NCAA Division 1 female track and field athletes. METHODS: Fifteen NCAA Division 1 track and field female athletes (Height = 165.20+7.85 cm; Weight = 61.11+9.46 kg; BF% = 18.80+4.92 %; Age = 19.80+1.57 years) were assessed for adequate squat form using the FMS deep squat protocol. Subjects then completed a dynamic warm-up before a wired EMG sensor was placed over the rectus femoris muscle belly of the right leg. Subjects completed two trials consisting of 10 repetitions of WBS with and without WBV, in a counterbalanced order. Root mean squared (RMS) values were collected using the EMG sensor during WBS trials. RMS values for WBS during each trial were analyzed using a Dependent t-Test with an alpha level of p < 0.05. RESULTS: Mean values for RMS were 74.92+22.81 µV for WBV trials, and 53.11+24.46 µV for ground squat trials. The values for RMS were significantly (p < 0.001) greater for the WBV trials. CONCLUSIONS: Significantly higher RMS values occurred for the WBV trials which may indicate that more motor units were recruited in the rectus femoris when the athlete was performing WBS and experiencing WBV. Future research may be required to determine if the current study’s results may apply to collegiate male track and field athletes.